Bacillus pasteurii, urease activity

Several structures of ureases are available (2). In all cases, the active site contains two Ni(II) ions bridged by the carboxylate group of a carbamylated lysine and by a hydroxide ion (Fig. lA). Each Ni is also coordinated by two histidines and one water molecule, whereas Ni(2) is further bound to an aspartate, resulting in a pentacoordinate Ni(l) and hexacoordinate Ni(2). In the resting state of the enzyme from Bacillus pasteurii, the active site accommodates a fourth water molecule, completing a tetrahedral cluster of solvent molecules (12). The access to the active site is regulated by a flexible helix-loop-helix motif, the position of other amino acids involved in the catalysis being also critically affected by the flap movement. 

Three Ni-confaintng enzymes (see Nickel Enzymes Cofactors) appear to utilize Ni metallochaperones for enzyme activation. UreE appears to function in NP+ delivery to urease. The Klebsiella aerogenes protein binds 6 Ni per dimer, whereas that from Bacillus pasteurii binds a single Ni per dimer. The metal content differences arise from a His-Asp-His sequence near the middle and a histidine-rich region at the carboxyl terminus of the former protein. Truncated K aerogenes UreE protein, missing the His-rich... 

Remaut H, Saeaeov N, Ciueli S and Van Beeu-MEN J (2001) Structural basis for Ni transport and assembly of the urease active site by the metal-lochaperone UreEfrom Bacillus pasteurii. J Biol Chem 276 49365-49370. 

Figure 2 The active site of native urease from Bacillus pasteurii (pdb 2UBP). Nickel atoms are in dark gray, carbon atoms in light gray, oxygen atoms in white, and nitrogen atoms in black. Hydrogen bonding...

Fig. 1. Structural models of urease from Bacillus pasteurii in the native state (panel A), and its complexes with /3-mercaptoethanol (BME panel B), acetohydroxamic acid (AHA panel C), phosphate (PHO panel D), diamidophosphate (DAP model E), and boric acid (B(0H>3 panel F). The Ni atoms in the active site are shown as filled circles.

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