Azospirillum brasilense proteins

Specific activity refers to nmoles of acetylene reduced per mg protein per minute at 30 C in the presence of saturating amounts of the other protein. Superscript numbers indicate fractions 1 (MoFe protein) or 2 (Fe protein). The nitrogenase components firom several other organisms including Mycobacterium flavum (Biggins et al., 1971), Azospirillum brasilense (Ludden et al., 1978), and Anabaena cylindrica (Tsai and Mortenson, 1978) have been separated and partly purified. 

Whether, this is AMP or not remains to be established. Av2 and Cp2 were relatively free of these compounds. Moreover the uv spectrum of Rr2 contained a shoulder at 269 which was absent from Av2 and Cp2. This evidence, together with the observation that Rr2 and the Fe protein from Azospirillum brasilense require an activating factor (Section II,E), implies that at least two distinct types of nitrogenase Fe proteins exist. 

The peptidic link present in proteins, = C— NH—(C=0)— must lead to a 1 1 ratio between the N and C—O concentrations. Figure 36 presents results obtained for a soil bacterium Azospirillum brasilense at different culture times. The correlation between the concentration of carbon responsible for the component at 287.9 eV... 

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