Azide, phosphorylation inhibitor

The following is a summary of the effects of oxidation and phosphorylation inhibitors in addition to those which have been cited above. In resting E. coli (86) the incorporation of S -methionine was inhibited by azide (0.005Af) 100%, 2,4-dinitrophenol (O.OOIM) 96%, and fluoride (0.02M) 100%. Malonate, at concentrations of 0.02, 0.01, and O.OOhiW respectively, inhibited the incorporation of glycine in fetal rat liver homogenate 75, 65, and 40% (137), and the incorporation of alanine. 

Many inhibitors of catabolic pathways cause a decrease in cellular heat dissipation. They are therefore valuable tools to indicate the sources of the dissipation and give clues to the relative importance of each pathway in overall metabolic activity (see reviews by Kemp, 1987, 1993 Monti, 1987, 1991). To give a few examples from these reviews, sodium fluoride is a classical inhibitor of glycolysis and it has been shown to substantially reduce heat dissipation by human erythrocytes, lymphocytes, neutrophils, and murine macrophages, indicating the contribution of this pathway to metabolic activity. Cyanide inhibits oxidative phosphorylation by mitochondria at the cytochrome c oxidase complex (site 3) and studies revealed that it decreased heat production in a mouse LS-L929 fibroblast cell line but had no effect on human erythrocytes and neutrophils and murine macrophages, all of which lack mitochondria. Sodium azide inhibits at the same site and so it should come as no surprise that it had no effect on human neutrophils and lymphocytes, but it did reduce heat production by lymphocyte hybridoma cells, which contain... 

Since sodium azide or oligomycin, inhibitors of mitochondrial oxidative phosphorylation, did not affect the Ca " release, it became generally accepted that the intracellular pool corresponds to the SR. 

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