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Avidin-Biotin Disadvantages

The only disadvantage to the use of NHS-biotin or sulfo-NHS-biotin is the lack of a long spacer group off the valeric acid side chain. Since the binding site for biotin on avidin and streptavidin is somewhat below the surface of the proteins, some biotinylated molecules may not interact as efficiently with (strept)avidin as when longer cross-bridges are used (Green et al., 1971 Bonnard et al., 1984). [Pg.511]

The strong binding between biotin and strept(avidin) is a disadvantage when a captured biotin or biotinylated molecule needs to be released. Release usually requires harsh conditions such as 6 M guanidine hydrochloride, pH 1.5... [Pg.77]

Recently, the original possibility to coordinate biotinylated compounds on copper-poly (pyrrole-NTA) films was reported [17]. This approach combines the advantages of the affinity systems biotin/(strept)avidin and NTA/Cu " /histidine, eliminating the disadvantages of each system, and thus constitutes a real advance in the field of non-covalent... [Pg.257]

See other pages where Avidin-Biotin Disadvantages is mentioned: [Pg.65]    [Pg.71]    [Pg.65]    [Pg.71]    [Pg.901]    [Pg.591]    [Pg.598]    [Pg.93]    [Pg.199]    [Pg.228]    [Pg.174]    [Pg.65]    [Pg.65]    [Pg.70]    [Pg.73]    [Pg.571]    [Pg.229]    [Pg.1175]    [Pg.303]    [Pg.152]    [Pg.254]    [Pg.386]    [Pg.152]    [Pg.7]    [Pg.410]    [Pg.533]    [Pg.639]    [Pg.313]    [Pg.7]    [Pg.311]    [Pg.167]    [Pg.106]    [Pg.414]   


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Avidin-biotin

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