Autoradiography of Thin-Layer Chromatograms

If several thin-layer chromatograms are to be evaluated, it is a good idea to mark the individual plates with fluorescent material which is clearly registered on the X-ray film (Firm 24). 

The film most commonly used is No-Screen Medical X-Ray Safety Film (Firm 52). The emulsion of this material is quite sensitive and the resolution [611] suffices for TLC requirements. Suitable films are manufactured by other firms also (Firms 1, 9, 75, 107). Supermix developer or s6me other X-ray film developer (Firms 1, 9, 75, 107) is 

1 lower compartment, containing a coated 20 x 20 cm plate on a platform (5)  

Generally, identical chromatograms of C-labelled compounds are left 1, 2, 4 and 8 days in contact with X-ray film. Autoradiographs are obtained in this Avay, in which the ratios of the amounts of the separated components are approximately correctly reproduced and also autoradiographs in which the main components yield photographically over-dense spots and minor contaminants also show up. A complete picture of the qualitative composition of the separated mixture is thus obtained. 

If exposures of several weeks are needed, the thin-layer chromatogram and film are kept in a refrigerator to prevent secondary photographic effects. 

---

Fig. 83. Container for autoradiography of thin-layer chromatograms [73] (Firm 7).

---