Attributes of Layers and Columns

Separations by column liquid chromatography (HPLC) and thin-layer chromatography occur essentially by the same physical processes. The two methods are often considered competitors when it would be more realistic to consider them complementary techniques. The favorable attributes of thin-layer chromatography providing for its coexistence alongside column liquid chromatography are summarized in Table 6.1 [1]. 

Separation of samples in parallel Low-cost analysis and high-throughput screening of samples requiring minimal sample preparation. 

Disposable stationary phase Analysis of crude samples (minimizing sample preparation requirements) Analysis of a single or small number of samples when their composition and/or matrix properties are unknown Analysis of samples containing components that remain sorbed to the separation medium or contain suspended microparticles 

Static detection Samples requiring postchromatographic treatment for detection Samples requiring sequential detection techniques (free of time constraints) for identification or confirmation 

Storage device Separations can be archived Separations can be evaluated in different locations or at different times Convenient fraction collection for multimodal column/layer chromatography 

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