Atomic emission spectroscopy group 1 metals

Atomic spectroscopy is a quantitative technique used for the determination of metals in samples. Atomic spectroscopy is characterized by two main techniques atomic absorption spectroscopy and atomic emission spectroscopy. Atomic absorption spectroscopy (AAS) is normally carried out with a flame (FAAS), although other devices can be used. Atomic emission spectroscopy (AES) is typified by the use of a flame photometer (p. 168) or an inductively coupled plasma. The flame photometer is normally used for elements in groups I and II of the Periodic Table only, i.e. alkali and alkali earth metals. 

Analysis of NROR by plasma emission spectroscopy reveals that the enzyme does not contain any metals in significant amounts (>0.1 g-atom/mol) and flavin appears to be its only prosthetic group. The enzyme is quite thermostable, with the time required for a 50% loss in catalytic activity when it is incubated at 80° and 95° of 12 and 1.6 hr, respectively. These values are determined by maintaining the purified enzyme (0.4 mg/ml) in 60 mM EPPS, pH 8.0) in serum-stoppered glass vilas at the desired temperature and periodically removing samples to determine residual BVNOR activity at 80°. 

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