Atomic Absorption Spectroscopy Fundamentals and Applications in Medicine

Atomic Absorption Spectroscopy Fundamentals and Applications in Medicine 

Spectroscopy measures and interprets phenomena of absorption, dispersion or emission of electromagnetic radiation that occur in atoms, molecules and other chemical species. Absorption or emission is related to the energy state changes of the interacting chemical species which characterise them, which is why spectroscopy may be used in qualitative and quantitative analysis. 

The Bouger-Lambert-Beer Law is fulfilled with limitations in molecular absorption spectrophotometry (Skoog et al., 2008). 

In 1953, the Australian Physicist Alan Walsh laid the foundations and demonstrated that atomic absorption spectrophotometry could be used as a procedure of analysis in the laboratory (Willard et al., 1991). The theoretical background on which most of the work in this field was based is due almost entirely to this author (Elwell Gidley, 1966). 

The spectra of atomic absorption of an element are made up of a series of lines of resonance from the fundamental state to different excited states. The transition between the fundamental state and the first excited state is known as the first line of resonance, being that of greatest absorption, and is the one used for analysis. 

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Atomic Absorption Spectroscopy Fundamentals and Applications in Medicine T ... 

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