Assay Design and Implementation

3-keto reductase step in ergosterol C4-demethylation in Botryotiniajuckdiana [36]. H owever, this compound has no activity in the S. cerevisiae ergosterol reporter assay and neither does it inhibit the growth of S. cerevisiae, suggesting that S. cerevisiae is not a good model system to discover novel inhibitors of 3-keto reductase. 

Tier 3 tests are normally used a try to confirm the mode of action or to generate additional information that will add value to the lead. A good example of this is in the use of strains of S. cerevisiae that have been engineered to over-express a protein of interest. Provided the compound has inhibitory activity against 

Compounds that have in vitro ICjq values 1-5 pM (depending on the assay), and have been demonstrated to be linked to effects on whole cells, are regarded 

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