Asperigillus oryzae

Recently, we exploited the unique spectroscopic properties of AW to probe the disulfide-coupled folding of the hirudin N-terminal domain 1-47 and the binding to its the target enzyme, thrombin [90]. Before chemical synthesis, the resolution of the commercially available enantiomeric mixture of AW was carried out by treating the racemic mixture with acetic anhydride and subsequent enantioselective deacylation with immobilized Asperigillus oryzae acylase-I to yield L-AW (Scheme 9-1). Purified L-AW was then reacted with 9-fluorenylmethoxycarbonyl chloride... 

The synthesis of 2-acetamido-4-0-(2-acetamido-2-deoxy-P-D-galactopyranosyl)-2-deoxy-D-glucopyranose using P-A -acetylhexosaminidases from Asperigillus oryzae and Jack bean with a p-nitrophenyl activated donor [23] (Figure 12.8). 

The inhibitory effect of maltose, maltotetraose and maltopentaose was also investigated in the hydrolysis of y-cyclodextrin with Asperigillus oryzae a-amylase. The results are shown on Figures 2, 3 and 4. 

A yeast or other starter prepared in Japan from rice inoculated with the spores of the mold Asperigillus oryzae and permitted to develop a mycelium. Koji is used for making miso from soybeans, a popular fermented food in Japan. 

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