Aspen Trembling, Populus tremuloides

Trembling aspen wood (Populus tremuloides Michx.) was reduced to a powder in a Hurricane Pulverizer. Samples were prepared from extracted pulverized wood by ball-milling for 10 days in a rotating ceramic mill equipped with an air-conditioning unit which prevented the temperature of the contents of the mill from rising above ambient. Digestion of the samples with cellulase was done with a mixture of Schizophyllum commune and Trichoderma reesei culture filtrates at 30°C for 3 days. 

Trembling aspen, Populus tremuloides, 22eRa Near uranium tailings plant vs. control site Leaves 42 DW vs. 11-15 DW 4... 

HARUTA, M., PEDERSEN, J.A., CONSTABLE, C.P., Polyphenol oxidase and herbivore defense in trembling aspen Populus tremuloides)-. cDNA cloning, expression, and potential substrates. Physiol. Plant, 2001,112, 552-558. 

PETERS, D.J., CONSTABEL, C.P., Molecular analysis of herbivore-induced condensed tannin synthesis cloning and expression of dihydroflavonol reductase from trembling aspen (Populus tremuloides). Plant J., 2002,32, 701-712. 

HARUTA, M., MAJOR, I.T., CHRISTOPHER, M.E., PATTON, J.J., CONSTABEL, C.P., A Kunitz trypsin inhibitor gene family from trembling aspen Populus tremuloides Michx.) cloning, functional expression, and induction by wounding and herbivory. Plant Molec. Biol, 2001,46, 347-359. 

The study area is situated near Rouyn-Noranda, about 600 km to the north-west of Montreal, Canada (48°14 N, 79°01 W). Three sites were sampled at a downwind distance of 0.5, 2 and 8 km from the Horne copper smelter. At each site, soil samples were collected under three trembling aspen Populus tremuloides Michx) of similar age (< 30 years old). The soils developed in postglacial lake sediments of silt texture to form Luvisols, according to the Canadian System of Soil Classification (Soil Classification Working Group, 1998). The extent of soil contamination received through the atmospheric deposition of metals represents the main difference between the three sites as other characteristics were kept constant (e.g. climate, parent material, slope, aspect, etc.). For a more detailed description of the sites, see Seguin et al. (2004). 

The genus Populus includes hardwood trees commonly referred to as poplars, cottonwoods, and aspen, which are found throughout North America, Europe, and northern Asia. They are particularly dominant in the boreal forest and in the parkland zones of Canada and the American midwest. Widespread North American species include P. balsamifera (balsam poplar), with a range from Western Alaska to northern Quebec and Newfoundland, and P. tremuloides (trembling or quaking aspen), which is found from Alaska to Mexico. Both are prominent species of the boreal forest of Canada. P. trichocarpa (black cottonwood) is found along the Pacific coast from California to Alaska. The range of P. tremula (European aspen)... 

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