Asn-linked glycans

For this purpose, Ag zeolite and Ag, developed by Garegg25 and Paulsen,26 respectively, have been widely employed (Scheme 7.6). Reactions using these activators have found practical use in synthetic studies on glycoprotein glycans and glycolipids. For instance, Paulsen reported the chemical synthesis of the core pentasaccharide structure 6 common to all types of Asn-linked glycans, using the Ag... 

Since all hydroxyl groups of the p-linked mannose residue can be distinguished, stereo- and regiocontrolled synthetic routes to essentially all types of Asn-linked glycans can be conceived. This aspect was demonstrated by the synthesis of... 

The primary structures of two Asn-linked glycans from bovine fibrinogen have been determined by methylation analysis and NMR spectroscopy, and were also shown to be dianteimary [57]. 

I. B. H. Wilson, R. Zeleny, D. Kolarich, E. Staudacher, C. J. M. Stroop, J. P. Kamerling, and F. Altmann, Analysis of Asn-linked glycans from vegetable foodstuffs widespread occurrence of Lewis a, core al,3-linked fucose and xylose substitutions, Glycobiology, 11 (2001) 261-274. 

Faye, L., Gomord, V., Fitchetter-Laine, A. C., Chrispeels, M. J. Affinity pinification of antibodies specific for Asn-linked glycans containing alpha 1,3 fucose or beta 1,2 xylose. Anal Biochem., 1993, 209, 104-108. 

Fucosylation of the Asn-linked GlcNAc is a common modification unique to A-linked glycans. In vertebrates, core FucT adds fucose in an al-6 linkage (59), whereas in plants and invertebrates al-3-linked fucose is observed (60). 

EPO Tetraantennary complex type at three Asn sites and one O-linked glycans. More O-linked glycans and less sialic acid than native form manifest conformational differences and hence in the potency One O-linked and three N-linked glycans. N-linked glycans are extensively branched and complex type structure having tetraantennary glycans [212,215]... 

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