Ascorbic acid oxidase copper free

Dopamine- -hydroxylase is another copper enzyme that plays a major function in the biosynthesis of norepinephrine (40). A protective catalyst, superoxide dismutase, has been described to catalyze the dismu-tation of the superoxide anion free radical (41). Other enzymes known to contain copper are the laccases, the phenol oxidases, and the ascorbic acid oxidases (12). 

Copper-Free Ascorbic Add Oxidase. A totally different specific ascorbic acid oxidase has been prepared from fungal spores. This enzyme is not sensitive to copper inhibitors but is inhibited by sulfhydryl reagents. It does not attack ascorbic acid analogs. Like the copper-containing enzyme, fungal ascorbic acid oxidase gives rates proportional to oxygen tension. 

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