Artifactual generation of free radical signals in myocardial tissue

Artifactual generation of free radical signals in myocardial tissue 

The ESR spectrum of aerobically perfused rat heart with the frozen tissue chopped shows a symmetrical one-line spectrum, whereas ground tissue resulted in the appearance of a multi-line signal, a semiquinone at g = 2.004 and a peroxyl radical at gj = 2.006 and gy = 2.04 (Fig. 2) [75], The fact that radicals can be generated artifactually by mechanical grinding renders this procedure unsuitable for studying radical production during myocardial ischemia and 

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