Arabidopsis 13.50 Cress

In 2008, based on sequence similarities to the known HNLs, A/eHNL and //Z>HNL [43], Kragl and coworkers identified a novel / -specific HNL from a noncyanogenic plant, Arabidopsis thaliana (mouse-ear cress). This is the first / -selective HNL, but contains an a//3-hydrolase fold [25]. 

Arabidopsis thaliana (mouse-ear cress) (Brassicaceae) Cysteine protease inhibitor (CPI) protein homologues (genes) (11-51 kDa proproteins) Cysteine protease inhibitor protein homologues [141- 146]... 

Arabidopsis tlialiana (mouse-ear cress) (Brassicaceae) Arabidopsis genes encoding - 8 kDa Potato type 1 serine protease inhibitors Trypsin (inferred) [438]... 

Arabidopsis lhaliana (mouse-ear cress) Brassicaceae) ATTI-1 ( 60 aa) (deduced sequence from chromosome 1) Trypsin (inferred) (R20-I21 ifDRKC N-terminal sequence cf. [21) [515, 516]... 

Taji, T. et al., 2004, Comparative genomics in salt tolerance between Arabidopsis and aRabidopsis-related halophyte salt cress using Arabidopsis microarray. Plant Physiol. 135(3) 1697-1709. 

The existence of sulfite oxidase in plants snch as Arabidopsis thaliana (wall or thule cress), tobacco, pea, spinach, barley, carrot, and poplar trees has now been confirmed, and the enzyme from A. thaliana has been structurally characterized. The homodimeric, peroxisomal enzyme lacks the heme domain observed in animal sulfite oxidases, making it the simplest Mo-MPT enzyme yet isolated from eukarya. The animal and plant sulfite oxidases have an overall 47% sequence identity. The detoxification of sulfite, produced in the assimilation of sulfate into sulfur-containing amino acids and membrane components (sulfatides) and from environmental sources (acid rain), is also the principal role of the plant enzymes. 

Arabidopsis thaliana (mouse-ear cress) Brassicaceae) ATTI-A (60 aa), ATTl-A (60 aa), ATTI-B (61 aa), ATTl-C (55 aa), ATTl-D (59 aa) (deduced sequences from chromosome 2 [2]) Trypsin (inferred), ATTI-A (R19-I20), ATTI-A (R19-120), ATTI-B (R19-120), ATTl-C (R19-120), ATTI-D (K19-F20) [144, 516)... 

When a BR biosynthesis inhibitor, brassinazole, was applied to Arabidopsis thaliana, high levels of ribulose-l,5-bisphosphate carboxylase-oxygenase proteins accumulated in the plastids of the cotyledons. These results suggest that brassinazole treatment in the dark induces the initial steps of plastid differentiation, which occur prior to the development of fhylakoid membranes. This is a novel presumed function of BRs [16]. Brassinazole treament also retards the development of secondary xylem in cress [17]. 

The remainder of the biosynthesis takes place in the peroxisomes. The substrate is imported by the cassette-transporter comatose (CTS). The hydrogenation of the endocyclic double bond is carried out by 12-oxophytodienic acid reductase this enzyme belongs to a small group of flavin-dependent oxidoreductases. In accord with the reaction mechanism, which was suggested for the related Old Yellow Enzyme of yeast, two hydrogen bridges from histidine (His-186 and His-189 in the 12-oxophytodienic acid reductase from mouse-ear cress Arabidopsis thaliana)) towards the carbonyl group polarise the double bond, so that a hydride of the reduced flavin cofactor can be transferred to C-10. The carbanion in the 11 -position is then protonated by Tyr-191. 

Wan, L. Ross, AR. Yang, J. Hegedus, D.D. Kermode, AR. (2007). Phosphorylation of the 12 S globulin cruciferin in wild-tyjje and abil-1 mutant Arabidopsis thaliana (thale cress) seeds. Biochemical Journal, Vol.404, No.2, Qime 2007), pp. 247-256, ISSN 0264-6021... 

C12H18O10 322.268 Widespread in the exudates of all plant species studied. Isol. from seeds of Lepidium sativum (garden cress) and Arabidopsis thaliana. Allelopathic agent. Increases fructose 2,6-biphosphate level in plants. Growth promoter. Oil, amorph. powder (as Na salt). [a]o +87.8 (c, 0.03 in D2O). Strictly the Na salt is named Lipodimoide. 

Arabidopsis thaliana Thale cress Brassicaceae Plant Encyclopedia (2012)... 

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