Applications of TLC in Chemical and Biochemical Investigations with Radioisotopes

Applications of TLC in Chemical and Biochemical Investigations with Radioisotopes 

TLC is ideally suited for analysing the products of radiochemical syntheses and isolation of labelled compounds from complex reaction mixtures. The method has proved its value, for example, in the preparation of H- or C-tagged fatty acids [628], cholesterol esters [416], glyceryl ethers [425], phospholipids [569] and steroids [328]. 

The autoradiograph in Fig. 96 shows the analysis of the intermediate products formed in a synthesis of several stages. Radioactively labelled chimyl alcohol was prepared in accordance with, the following scheme 

The composition of all the intermediates, including recrystallised compounds and their filtrates, was determined [425] by scintillation counting of scraped-off zones [647]. The amounts needed for the analysis did not impair the yield (40%). 

It has likewise been possible to separate and analyse mixtures of water-soluble labelled substances, using TLC. An example is the I-labelled S-sulphonated A- and B-chains of insulin, using TLC on silica gel G or Amberlite IR-120 [435]. 

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