Application of the HPLC method

APPLICATION OF THE HPLC METHOD 8.1. Detection of abnormal lipoproteins 

The detection of abnormal lipoproteins can be done more effectively from the elution patterns monitored by protein or choline-containing phospholipids for the isolated lipoprotein fraction by the ultracentrifugation. In Fig. 20, the elution patterns of choline-containing phospholipids for each ultracentrifugal fraction are presented for three subjects normal female and patients with acute hepatitis and lecithin cholesterol acyltransferase deficiency. In contrast to normal subjects, all lipoprotein fractions for two pathological cases show the existence of abnormal lipoproteins in which a correlation between the particle size and density dose not exist. 

G4000SW+G3000SW. Sample serum of normal female (a), patients with liver cirrhosis (b), acute hepatitis (c), primary biliary cirrhosis (d), intrahepatic cholestasis (e) and drug induced injury (f). Elution positions and other HPLC conditions as in Fig. 14. 

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One of the first applications of the HPLC method was the investigation of differences in toxin profiles between shellfish species from various localities ( ). It became apparent immediately that there were vast differences in these toxin profiles even among shellfish from the same beach. There were subtle differences between the various shellfish species, and butter clams had a completely different suite of toxins than the other clams and mussels. It was presumed that all of the shellfish fed on the same dinoflagellate population, so there must have been other factors influencing toxin profiles such as differences in toxin uptake, release, or metabolism. These presumptions were strengthened when toxin profiles in the littleneck clam (Prototheca Staminea) were examined. It was found that, in this species, none of the toxin peaks in the HPLC chromatogram had retention times that matched the normal PSP toxins. It was evident that some alteration in toxin structure had occurred that was unique in this particular shellfish species. 

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