Application of PAMPA for Drug Discovery

In addition to screening compounds, PAMPA was recently applied for high-throughput formulation screening. For early in vivo toxicity studies, it is often necessary to rapidly develop an effective formulation to increase exposure. Such formulations are usually a combination of several excipients. Simultaneous assessment of solubility and permeability is required since formulation excipients may reduce the apparent permeability by reducing the free fraction of a drug [68]. 

PAMPA data were also used to understand permeation into the pharmacological and toxicological target cells. A discrepancy between the enzyme level assay and cell-based assay is often observed in drug discovery, resulting in a misreading of the structure-activity relationship [69]. 

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