Appearance of cells and laboratory cultures

Pectinatus cells are nonspore-forming, slightly curved helical rods, 0.4-1.0 by 2-50 pm or more, with rounded ends. They typically occur singly, in pairs or rarely in short chains. In older cultures elongated snake-like cells and various round cell formations can be seen (Haikara Juvonen, 2009). A distinctive feature of the Pectinatus 

Cultural characteristics of Pectimtus species have been studied in peptone yeast extract fructose (PYF) medium (http //culturecollection.vtt.fi) and MRS medium. The beer-spoilage species form circular, entire, glistening and opaque colonies on PYF 

Selenomonas lacticifex and the beer-spoilage Propionispira species are motile rodshaped bacteria. They may lose their mobility upon repeated cultivations. None of these species forms endospores. The cells of S. lacticifex are curved cresceut-shaped 

Cultural characteristics have been determined on modified MRS agar medium in which the beer-spoilage Propionispira species form circular, smooth, opaque and slightly yellow colonies with a diameter of 1-2 mm after 3 days of incubation (Schleifer et al., 1990). The colonies of S. lacticifex are also smooth, circular, opaque and yellowish with a diameter of 2-3 mm after 3 days of incubation. 

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GPRT- defective cells appear in population. Using the method of the selective pressure by 5-BDU and 8-Ag, the mutant cultures of cells, defective by the indicated enzymes, are obtained. The intraspecific and interspecific hybrid cultures of cells are firstly obtained in the laboratory of the cellular biotechnology, A4H (SPEV TK- x lymphocytes of horse) and others. Hybrid cultures possess the unique properties. They are the ability to grow in the monolayer and in suspension, the high sensitiveness to the viruses. Hybrid cultures PO-TK-KHLO, PO-TK-KHSO are sensible to the agent scrappy, what allows using them for the study of diseases, caused by prions. 

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