Electrophoretic mobility apparent

The migration in CE is obviously influenced by both the effective and the electroosmotic mobility. Therefore, the proportionality factor in the relationship of the migration velocity and the electric field strength in such a case is called the apparent electrophoretic mobility (/iapp) and the migration velocity the apparent migration velocity (vapp). The /iapp is equal to the sum of /migration velocity is expressed as... 

The overall migration in CE is determined by the combined effect of the effective and the electroosmotic mobility. The apparent electrophoretic mobility is therefore used as the... 

The apparent electrophoretic mobility /iapp is defined by an equation analogous to (8.1) or (8.2) such that ... 

The efficiency is therefore directly proportional to the the applied voltage (V) and inversely proportional to the diffusion coefficient of the analyte (D). The sum of contributions from electoosmotic flow (/ ,) and the electrophoretic mobility (fiel) result in the overall, or apparent, electrophoretic mobility of the analyte ... 

The apparent electrophoretic mobility of an analyte in micellar electrokinetic chromatography depends on three factors the electroosmotic mobility for the system the fraction of analyte in the electrolyte solution and its electrophoretic mobility and the fraction of analyte in the pseudostationary phase, and the electrophoretic mobility of the micelles (assuming that the mobility of the analyte-micelle complex is the same as the micelle). If we introduce the chromatographic retention factor, defined as the ratio of the number of analyte molecules in the pseudostationary phase to the number in the... 

The resolution, R, between two ions is dependent on the difference in electrophoretic mobility between the two species A/j.ep (i.e. the separation selectivity), the applied separation voltage V, the apparent electrophoretic mobility, tiapp, and the diffusion coefficient D ... 

In principle, field-enhanced stacking is a technique for the concentration of charged analytes using discontinuous solutions in the capillary. Neutral analytes are not concentrated by this method because they do not have electrophoretic mobility. However, by utilizing charged micelles, an apparent electrophoretic mobility can be imparted to a neutral analyte according to the principles of MEKC. As in conventional field-enhanced sample stacking, sample solutions are prepared in a... 

The electrophoretic mobility of a peptide, determined relative to the EOF or to a standard, can be used for identification. Because at pH 2.5 the EOF is virtually suppressed and difficult to determine experimentally, (26) the relative electrophoretic mobilities of the nine peptides were determined using an internal standard, Dynorphin A fragment 1-13. The apparent mobility (p pp) of the reference peptide Dynorphin was found to be 3.05 x 10" ( Dyn== L x L/1 x V Ld=40 cm, Lt=47 cm, t=274.26 sec, V= 22500 Volts). For each peptide the apparent electrophoretic mobility was then determined experimentally and an electrophoretic mobility relative to the reference peptide calculated ( Tt = l app Poyn ) These values are given in Table 1. 

Figure 6.15. Apparent electrophoretic mobility values obtained at diRerent cell positions.

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