Apparatus for isotachophoresis

Instead of capillary (narrow bore tube) isotachophoresis, this type of electro-migration separation can be materialized on cellulose acetate strips [173]. Combinations with classical electrophoresis resulting in two dimensional separations have also been described [174,175], 

Isotachophoresis of proteins can also be done in dilute polyacrylamide gel, in which sieving effects are oppressed. The results are comparable with isoelectric focusing or disc electrophoresis. The isotachophoretic profile is analogous to the isoelectric focusing pattern this is because proteins are separated mainly according to their p7 values. Chrambach [138] has shown that the advantage of isotachophoresis over isoelectric focusing is that, in isotachophoresis, no precipitation of proteins takes place. Carrier ampholytes were applied by Svendsen [128] to separate protein zone. 

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