Antisense oligonucleotides structure

Semple SC, Klimuk SK, Harasym TO, et al. Efficient encapsulation of antisense oligonucleotides in lipid vesicles using ionizable aminolipids formation of novel small multilamellar vesicle structures. Biochim Biophys Acta 2001 1510 152. 

Mishra, R.K., Le Tinevez, R. and Toulme, J.J. (1996) Targeting nucleic acid secondary structures by antisense oligonucleotides designed through in vitro selection. Proc. Natl. Acad. Sci. USA, 93, 10679-10684. 

Mishra, R.K. and Toulme, J.J. (1994) In vitro selection of antisense oligonucleotides targeted to a hairpin structure. 

Lambert, R. C., Maulet, Y., Dupont, J. L., et al. Polyethylenimine-mediated DNA transfection of peripheral and central neurons in primary culture Probing Ca2+ channel structure and function with antisense oligonucleotides. Mol. Cell. Neurosci. 7 239-246, 1996. 

Following a short introduction, the book is structured into three sections The Basics section discusses individually the pharmacokinetics of peptides, monoclonal antibodies, antisense oligonucleotides and gene delivery vectors. The subsequent Challenges and Opportunities section includes more detailed considerations on selected topics, including technical challenges such as bioanalytical... 

A key to understanding antisense technol-t ogy is to consider it in a pharmacological context. It is essential to understand the structure, function, and metabolism of the receptors for these drugs. As with any of the class of dmgs, it is essential to consider the effects of antisense oligonucleotides in the context of dose-response curves. It is essential to consider the future in the context of advances in antisense biology and medicinal chemistry that result in improved pharmacological behaviors. 

In subsequent studies, we evaluated the in-I teractions of antisense oligonucleotides with boh structured and unstructured targets, and the impacts of these interactions on RNase H in more detail (150). Using a series of non-cleavable substrates and Michaelis-Menten... 

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