Antigens neoglycoproteins

Because O-glycosylation can also be accomplished with active esters (e.g., penta-fluorophenyl esters) [11] of Fmoc serine and threonine, the Fmoc technique provides a general method for the synthesis of glycopeptides. Thomsen-Friedenreich antigen glycopeptides and neoglycoproteins have been obtained by this method in preparative amounts [12], In combination with acid-labile polymeric benzyl ester anchors, this Fmoc technique was applied to solid-phase syntheses of glycopeptides [11,13,14]. 

The specificity of the antiserum raised against the Lea neoglycoprotein (9) was determined by hapten-inhibition studies using synthetic oligosaccharides,72 and derivatives of bovine and horse serum-albumin to which the Lea trisaccharide had been attached.73 On the basis of hapten inhibition of precipitation, this antiserum was shown to be highly specific for the carbohydrate portion of the synthetic antigen,... 

The carrier proteins themselves were usually immunogenic, although the antibodies they elicited could be readily adsorbed out of the antisera. In some instances, the contribution of the protein to the specificity of the immune serum raised against the neoglycoprotein was evaluated by attaching the carbohydrate antigen to different carrier-proteins.73... 

G. O. Aspinall, N. K. Khare, R. K. Sood, D. Chatterjee, B. Rivoire, and P. J. Brennan, Structure of the glycopeptidolipid antigen of serovar 20 of the Mycobacterium avium serocomplex, synthesis of allyl glycosides of the outer di- and tri-saccharide units of the antigens of serovars 14 and 20, and serology of the derived neoglycoproteins, Carbohydr. Res., 216 (1991) 357-373. 

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