Antigen or Hapten Conjugation to Liposomes

Protocol for the Encapsulation of Antigen into Liposomal Vesicles 

Prepare a homogeneous lipid mixture by dissolving the desired components in chloroform. A suggested recipe may be to use a mixture of DPPC DPPG choles-terol at a molar ratio of 7.5 2.5 5. Evaporate the chloroform using a rotary 

Dissolve the hapten or antigen to be encapsulated at a concentration of 21 ptmol/ml in degassed, nitrogen-purged 10 mM Hepes, 0.15 M NaCl, pH 6.5. 

Protocol for the Coupling of Peptide Haptens Containing Sulfbydryl Groups to Liposomal Vesicles 

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