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Antibody-deglycosylated ricin A chain

Dissolve deglycosylated ricin A chain (dgA) in 0.1 M sodium phosphate, 0.15 M NaCl, 10 mM EDTA, pH 7.5, at a concentration of 10 mg/ml. The buffer should be degassed under vacuum and nitrogen bubbled through it to remove oxygen. Prepare half the amount of A-chain solution as the amount of antibody prepared... [Pg.531]

Ghetie, V., Ghetie, M.-A., Uhr, J. W., and Vitetta, E. S. (1988) Large scale preparation of immunotoxins constructed with the Fab fragment of IgGl murine monoclonal antibodies and chemically deglycosylated ricin A chain. /. Immunol. Methods 112, 267—277. [Pg.708]

See other pages where Antibody-deglycosylated ricin A chain is mentioned: [Pg.841]    [Pg.531]    [Pg.511]    [Pg.841]    [Pg.531]    [Pg.511]    [Pg.830]    [Pg.839]    [Pg.843]    [Pg.246]    [Pg.521]    [Pg.528]    [Pg.393]    [Pg.501]    [Pg.508]   


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Antibody-deglycosylated ricin

Antibody—deglycosylated

Deglycosylation

Ricin

Ricin A chain

Ricin A chain deglycosylated

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