Antibodies cystamine

Using this approach, EGF has been successfully conjugated by disulfide exchange to the A chain of diphtheria toxin (Shimisu et al., 1980). A cystaminyl derivative of insulin also could be conjugated to the A chain of diphtheria toxin by this method (Miskimins and Shimizu, 1979). Other references to disulfide exchange using cystamine include Oeltmann and Forbes (1981) and Bacha et al. (1983) who prepared antibody-toxin and peptide-toxin conjugates, respectively. 

Figure 21.10 Cystamine may be used to make immunotoxin conjugates by a disulfide interchange reaction. Modification of antibody molecules using an EDC-mediated reaction creates a sulfhydryl-reactive derivative. A-chain toxin subunits containing a free thiol can be coupled to the cystamine-modified antibody to form disulfide crosslinks.

C.C. Wang, H. Wang, Z.Y. Wu, G.L. Shen, and R.Q. Yu, A piezoelectric immunoassay based on self-assembled monolayers of cystamine and polystyrene sulfonate for determination of Schistosoma japoni-cum antibodies. Anal. Bioanal. Chem. 373, 803-809 (2002). 

Other reagent systems can be used to form disulfide linkages between antibody and toxin molecules in immunotoxin conjugates. Cystamine can be incorporated into... 

Antibody Containing Cystamine Cystamine Activated Antibody... 

Lee and Chang [126] used a cystamine SAM to immobihse antibodies to develop a sensor for the detection of bovine ephemeral fever virus. The im-optimised sensor was used in a flowing solution and detected the antigen in real time to 5 xgmL Positive results were observed in bovine sera samples. 

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