Antibodies as nanopatterning reagents

Positional control of conjugation is essential at two levels in the assembly of nanobiological devices [6-8], Recently developed chemoselective conjugation 

Monoclonal antibodies to sulfhydryl-terminated PAMAM dendrimers [9,10] could be bound to sulfhydryl-terminated PAMAM dendrimers under stoichiometric conditions favoring complexes of one antibody to one dendrimer. After binding, the exposed sulfhydryl surface may be derivatized to eliminate SH groups. Antibody and dendrimer complexes are then dissociated and the patterned dendrimers are isolated. Only areas masked by the antibody retain sulfhydryl groups, and can therefore be differentially derivatized (to a gold adduct, or to maleimide-linked macromolecules, for instance). 

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