Anti-P-selectin

Recently, monoclonal antibodies were attached to gas-filled microbubbles using this spacer coupHng technology. Testing in vitro, in a cell culture system, demonstrated selective accumulation of decafluorobutane-based lipid shell MP1950 microbubbles with covalently attached anti-lCAM-1 antibodies, onto the surface of activated endotheUum [8]. Anti-P-selectin antibodies attached to such microbubbles via an avidin-biotin scheme showed selective accumulation in the areas of inflammation and ischemia/reperfusion injury in an animal model [93]. In the latter example, biotin was also connected to the microbubble surface via a flexible polymer spacer arm. 

Rolling of cells (neutrophils) initiated by cell adhesion was studied in a Y-channel. Inhibition by various anti-cell-adhesion molecules (anti-E-selectin, anti-P-selectin, sialyl Lewis X, Furoidan) was studied. Comparison can easily be made for cells with rolling and without rolling (control) in the two arms of the Y-channel [881],... 

Anti-VEGF, anti-bFGF, anti-PDGF, anti-L-selectin, anti-P-selectin, anti-neutrophil elastase, anti-thrombin... 

Selectins include P-selectin (platelet selectin), E-selectin (endothelial cell selectin), and L-selectin (leukocyte selectin). P-selectin enables binding of platelets, polymorphonuclear leukocytes, and monocytes to activated endothelial cells and of leukocytes to activated platelets. P-selectin is expressed in the kidneys in systemic lupus erythematosis [266, 267]. The up-regulation of P-selectin expression in glomerui following binding of anti-GBM antibody may be an... 

Mayadas TN, Mendrick DL, Brady HR, Tang T, Papayianni A, Assmann KJ, Wagner DD, Hynes RO, Co-tran RS (1996) Acute passive anti-glomemlar basement membrane nephritis in P-selectin-deficient mice. KidneyInt 49 1342-1349... 

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