Angle rotor

Free subcellular particles or virus from Percoll by centrifuging the respective fraction of cell fractionation in a fixed-angle rotor at 100 000 X g for 90 min. Percoll forms a solid pellet whidi contains practically no material of interest. 

Operation of a fixed angle rotor. A Loading of sample. 

Operation of a fixed angle rotor A Loading of sample B Sample at start of centrifugation C Bands form as molecules sediment D Rotor at rest showing separation of two components... 

Figure 10.5 Pore water extraction by (A) drainage centrifugation using a swing-out rotor and (B) immiscible liquid displacement using a fixed-angle rotor (after Kinniburgh and Miles, 1983).

Beckman Instruments. (1988). Instructions for Using the JA-10 Fixed Angle Rotor. Beckman J-21 Series, J—6, andJ-6B. Spinco Division of Beckman Instruments, INC., Palo Alto, CA. 

Add sterile water to the neutralized ssDNA solution in the Centricon-100 tube to a total volume of 2.0 ml. Spin for 25 min in a fixed-angle rotor at the highest speed recommended for the Centricon tubes. Add... 

Thermostatized centrifuge with fixes angle rotor (angle of inclination 33°) (e.g. Hettich Rotixa RP 001260 Tuttlingen Germany)... 

Figure 4-36. Fixed angle rotor. On the left is illustrated a conventional centrifuge tube, in which liquid is pressed against the lid during centrifugation. The right depicts a tube with an offset neck to avoid this problem.

Example. Calculate the RCF exerted at the top and bottom of a sample vessel spinning in a fixed angle rotor such as that shown in Figure 9-1. Assume that the rotor dimensions, r in and rma, are 4.8 and 8.0 cm, respectively, and that it is spinning at a speed of 12,000 rpm. By equation 4,... 

Figure 9-9. Various configurations of angle rotors for the preparative ultracentrifuge. (Courtesy of Beckman Instruments, Palo Alto, Calif.)...

The experiments were carried out in 250-mL graduated cylinders with ground joints at room temperature (20—22 °C). Centrifuge K24 (Janetzki, GDR) was used for the centrifugation of 25 mL samples. The minimum" and the maximum diameters of its angle rotor were 5 and 14 cm, respectively. At 17,000 r.p.m. the g value for the 7-cm diameter was 15,350 g. Aluminium concentrations were determined colorimetrically with aluminon . Colour was determined spectrophotometrically at 387 nm with the correction for turbidity at... 

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