Androstanediols

Selected ion monitoring GC/MS trace of the HFBA derivatives of nine neuroactive steroids found in human plasma. The separations were accomplished on a Restek Rtx-200 MS column. Relative absorbance was measured at negative Cl for m/z values of 474 (pregnanolones), 492 (pregnenolone), 664 (androstanediols), and 706 (pregnanediolones). (Everhart et al., unpublished)... 

Fig. 2.2. Flowdiagram of the method for the simultaneous determination of urinary androstanediols and pregnanediols.

Androstanediol Testosterone Silica gel F254 Benzene-ethyl acetate (1 1) -Hexane-ethyl acetate (1 1) 20... 

The possibility of preliminary isolation of substances under analysis in the form of hydrazones is frequently utilized in the determination of ketosteroids in complex mixtures such as biological materials. In this form they are even sometimes subjected directly to GC analysis. Charransol et al. [378] determined androstanediol and testosterone in urine. After hydrolysis the sample was extracted with methylene chloride, evaporated and treated with Girard T reagent. Free hydroxysteroids were extracted with diethyl ether and... 

Meikle AW, Odeh WD. Effect of short- and long-term dexamethasone on 3a-androstanediol glucuronide in hirsute women. Fertil Steril 1986 46 227-31. 

Horton R, Lobo R, Hawks D. Androstanediol glucuronide in plasma, a marker of androgen action in men and women. Clin Res 1982 30 491A. 

Pang S, Wang M, Jeffries S, Riddick L, Clark A, Estrada E. Normal and elevated 3 alpha-androstanediol glucuronide concentrations in women with various causes of hirsutism and its correlation with degree of hirsutism and androgen levels. J Clin Endocrinol Metab 1992 75 243-8. 

Rao J, Sayjani G> Petersen JR. Radioimmunoassay kit for the quantitative measurement of androstanediol glucuronide in unextracted serum. Clin Chem 1991 37 1049. 

Testosterone is metabohzed in the liver to androsterone and etiocholanolone (Figure 58-3), which are biologically inactive. Dihydrotestosterone is metabolized to androsterone, androstane-dione, and androstanediol. 

Iodosylbenzene has been used as an effective oxidant in hydrocarbon hydroxylation catalyzed by metal-loporphyrins [687-696]. In particular, various iron(III) and manganese(in) porphyrins can be used as catalysts in hydroxylations of cyclohexane, cyclohexene, adamantane and aromatic hydrocarbons [687,688, 692]. Breslow and coworkers have reported regioselective hydroxylations of several steroidal derivatives catalyzed by metalloporphyrins [689-691]. In a specific example androstanediol derivative 644 was... 

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