Analytical Speed, Selectivity and Quantitation Limits

Two approaches are generally used to develop methods with lower detection and quantitation limits for target compounds. One approach involves the use of sample cleanup methods such as size exclusion chromatography (SEC) for the 

To reduce costs of sample analyses, efforts are often directed toward increasing analytical speed (e.g., MaStovskd and Lehotay, 2003). Generally, several tradeoffs result from increased speed of analysis, which can be illustrated as follows  

The use of sophisticated instrumental systems such as high-resolution GC-MS does not guarantee satisfactory quantitation of the hundreds of chemicals sometimes present in SPMDs without some fractionation of sample residues. Thus, the complexity of target residues, as well as interferences from the matrix sampled can be determinants in the cleanup and separation procedures needed for satisfactory analyses. The following discussion presents the salient features of the typical processing and analytical procedures applied to SPMD samples. 

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