Fiber optic fluorescent sensors analytical probe

In particular optical sensing systems belong to this group, which are described in detail in Chapter 27. For example, fluorescent reporter groups are incorporated into the MIP, the properties of which are altered upon analyte binding [14-16]. A very sensitive sensor for a hydrolysis product of the chemical warfare agent Soman has been described based on a polymer-coated fiber optic probe and a luminescent... 

Fig. 5 - Displacement of the fluorescent probe FHMI by the imidazolinone and non-imidazolinone compounds, (left) Chemical structures of three imidazolinone class compounds and three non-imidazoline agrochemicals used to demonstrate the specificity of the fiber optic sensor, (right) Reduction of steady-state fluorescence by the various analytes. A 25 nM FHMI in casein/PBS solution was perfused to reach a steady state before a solution of 1 jlM compound was added to the FHMI, casein/PBS. Plot 1, imzamethabenz methyl 2, imazapyr 3 imzaaquin 4, chlorimuron ethyl 5, primisulfuron 6, sethoxydim. Reproduced with permission from reference 2, Copyright 1993 American Chemical Society.

Fiber-optic biosensor can be potentially used for clinical sample analysis. As described above, by using enzymes and antibodies immobilized on the fiber tip or around the fiber core (evanescent field), different fiber-optic biosensors have been developed. In most cases, the measurement is based on changes in fluorescence intensity. The target analytes include glucose, cholesterol, enzymes, antibodies, bacteria, and viruses. Measurements are usually fast and simple, and in many devices, the probe is disposable however, the instability of the biological recognition materials reduces the sensor lifetime. 

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