Analytical HPLC temperature silica capillaries

Glycerin is used in Nasonex primarily as a humectant. For its quantification, both capillary gas chromatography method and HPLC methods may be selected. The GC is equipped with a flame-ionization defector, a 0.53 mm x 30 m fused silica analytical column coated with 3.0-p,mG43 stationary phase, and a 0.53 mm x 5 m silica guard column deactivated with phenylmethyl siloxane. The carrier gas was helium with a linear velocity of about 35 cm/s. The injection port and detector temperature was maintained at 240 and 260°C, respectively. The injection mode is splitless. The column temperature is programmed to be maintained at about 40°C for 20 min, then to increase to 250°C at a rate of 10°C/min and to hold at 250°C for 15 min. 

---