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Analytical HPLC of Hapten-Protein Conjugates

Reversed-phase (rp-) or hydrophobic (HIC) HPLC is an excellent tool for analysis of proteins and peptides. Especially phenyl media, e.g., TosoHaas TSK-Gel Phenyl-5PW-RP, fit well [Pg.108]

Use an HPLC apparatus with gradient system, pre-column and 5 X 0.5-cm separation column is used. Equilibrate the column with Soln. A at flow rate of 0.8-1.0 ml/min. [Pg.108]


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Conjugation of proteins

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Haptenation

Haptene

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Protein conjugates

Protein conjugation

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