Applications of enzymes, analytical

Enzymes as analytical reagents. Although the technological applications are quantitatively the more important, many of the analytical applications of enzymes are unique and are becoming increasingly important. 

Thus far, few analytical applications of enzymes in a nonaqueous solvent are available. One example is the use of oxidases to oxidize hydrophobic substrates. 

Analytical Applications of Enzymes RELATIVE RATE OF HYDROLYSIS... 

I. Karube, Analytical Application of Enzymes Enzyme Sensors for Clinical, Process, and Environmental Analyses, in J. E. Kennedy (Ed.), Biotechnology. VCH, Weinheim, 7a (1987) 685-708. 

We have recently begun studies geared at the analytical application of enzymes immobilized on microelectrodes. We had previously demonstrated that 4-amino phenyl phosphate could be employed as a substrate for the determination of the enzymatic activity of alkaline phosphatase by following the oxidation of 4-amino-phenol which is the product of the enzymatic reaction [28]. In the present case, the interest was in determining whetiier such an approach could be transposed to a microelectrode and to determine if the immobilization process gave rise to an enzymatically active interface. 

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