Analysis of Body Fluids after Extraction with Solvents

Analysis of Body Fluids after Extraction with Solvents 

Infrared analysis can be applied to extractions of body fluids for the following classes of compounds (a) halogenated hydrocarbons (b) alcohols (c) ethers and aldehydes and (d) ketones. Most of the 160 substances (except the gases) given in the table of Stewart and Erley (1965) referred to earlier may be extracted from body fluids and analyzed by infrared methods. 

The process of extraction can be enhanced for volatile compounds that are soluble in both water and the solvent by the addition of an anhydrous salt, or by adjustment of the pH of the specimen based on knowledge of the pK of the organic compound. 

For sampling purposes, direct injection of blood through a self-sealing rubber stopper is not recommended because many volatile organic compounds can penetrate rubber stoppers, thus affording possible contamination from the surrounding atmosphere. 

Feldstein (1965a) has also described the analysis of toxic gases in blood. A rapid simple analysis involved aeration of acidified samples of blood into a 10-m path- 

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