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An Affinity-Based ELISA Assay to Identify Potent Binders

An Affinity-Based ELISA Assay to Identify Potent Binders [Pg.45]

ELIS As are widely used biochemical assay techniques to identify binding partners. Generally, one assay component is immobilized on a solid support, while a soluble [Pg.45]

Evaluation of Cell Permeability and Cellular Activity by Monitoring Insulin Receptor Signaling [Pg.47]

For this purpose, mammalian cells were incubated with the self-silenced peptide analog (13) introduced in Section 3.2.2 as well as the prodrug (15) introduced in Section 3.2.3, and subsequently treated either with or without insulin. [Pg.47]

We further introduced ways to achieve cell membrane permeability using the example of the negatively charged bivalent PTPIB inhibitor [20, 22]. The question [Pg.47]




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Affinity assays

Affinity-based

Based Assays

ELISA

Potent

Potentization

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