Amylase fungal, assay

Lipase (Microbial) Activity for Medium- and Long-Chain Fatty Acids, (S3)105 Lysozyme Activity, (S3)106 Maltogenic Amylase Activity, 804 Milk-Clotting Activity, 805 Pancreatin Activity, 805 Pepsin Activity, 807 Phospholipase A2 Activity, 808 Phytase Activity, 808 Plant Proteolytic Activity, 810 Proteolytic Activity, Bacterial (PC), 811 Proteolytic Activity, Fungal (HUT), 812 Proteolytic Activity, Fungal (SAP), 813 Pullulanase Activity, 814 Trypsin Activity, 814 Enzyme Assays, 786 Enzyme-Hydrolyzed (Source) Protein,... 

Hyaluronidase is involved in bacterial and fungal infections because of virulence factors evoked by tissue degradation and mediates host-pathogen interactions [47]. Since hyaluronic acid (HA) is a major component of the extracellular matrix involved in joint lubrication, a sensitive hyaluronidase assay is important. Current hyaluronidase assays rely on turbidimetric techniques that require high levels of the enz3Tne and are relatively inaccurate [47]. HA was previously shown to bind cyanines [48,49[. The detection scheme designed for CMA, CMC, and amylase enzyme described earlier was also applicable to HA and hyaluronidase activity [19]. Scaffold Destruction ... 

The Perkin-Elmer model 191 grain amylase analyser has potential usefulness to the cereal industry for measurement of amylase.For example, it can satisfactorily measure the potency of fungal a-amylase supplements, With amylo-pectin as substrate, it can measure various concentrations of a- or )3-amylase. The analyser cannot, however, differentiate between the effects of these two enzymes, which are both normally present in cereals. This limits its present usefulness, particularly for measurement of a-amylase. Selective inactivation with HgClj or heat-treatment cannot guarantee that /3-amylase is completely destroyed or that a-amylase has not been affected. Manufacturer refinements to the prototype method and instrument such as developing a /3-limit dextrin substrate and increasing the sensitivity of the analyser may ultimately provide a simple, rapid assay of a-amylase. 

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