Amplified Ribosomal DNA Restriction Analyses

Infrared spectroscopy, including FTIR Polyacrylamide gel electrophoresis (PAGE) Amplified ribosomal DNA restriction analysis (ARDRA)... 

Ventura, M., Casas, I.A., MoreUi, L., Callegari, M.L. (2000). Rapid amplified ribosomal DNA restriction analysis (ARDRA) identification of Lactobacillus spp. isolated from faecal and vaginal samples. Syst. Appl. Microbiol, 23, 504—509. 

Roy, D. and Sirois, S., Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the Idh gene, FEMS Microbiol. Lett., 191, 17, 2000. 

Genotyping of individual isolates by ARDRA (Amplified Ribosomal DNA Restriction Analysis)... 

ARDRA Amplified ribosomal DNA restriction analysis BAG Bacterial artificial chromosome... 

Fig. 9 Principle of amplified ribosomal DNA restriction analysis (ARDRA). The example demonstrates the use of ARDRA in the screening of three bacterial strains (A-C). Two restriction enzymes are used (designated as X and Y)...

Coton et al. (2005b) developed a PCR-based amplified ribosomal DNA restriction analysis method for rapid detection of Zymomonas at the subspecies level. A further duplex PCR method with primers specihc for 23S rRNA gene for detection of Zymomonas species has been developed (Coton et al., 2005b). This method could detect Zymomonas species within 24 h with sensitivity of 10 CFU/ml (Coton et al., 2005b). Coton, Laplace, Auffray, and Coton (2006) characterised several strains of Z. mobilis with random amplified polymorphic DNA (Table 8.3). 

Guan, L.L., Hagen, K.E., Tannock, G.W., et al. (2003) Detection and identification of Lactobacillus species in crops of broilers of different ages by using PCR-denaturing gradient gel electrophoresis and amplified ribosomal DNA restriction analysis. Appl Environ Microbiol 69, 6750-6757. 

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