Amplicon length

Suzuki, M. T., Rappe, M., and Giovannoni, S. J. (1998). Kinetic bias in estimates of picoplankton community structure obtained by measurements of smaU-subunit rRNA gene PCR amplicon length heterogeneity. Appl. Environ. Microbiol. 64, 4522—4529. 

TABLE 7.1 Primer-Probe Systems Used and Amplicon Length Expected"... 

Moreno, L., Mills, D., Entry, J., et al.. Microbial metagenome profiling using amplicon length heterogeneity-polymerase chain reaction proves more effective than elemental analysis in discriminating soil specimens, J Forensic Sci, 51, 2006. 

Forward Primer Reverse Primer Occurrence of Primer Pair in All Bacterial Sequences Occurrence of Primer Pair in Enteric Sequences Average Amplicon Length and Std. Dev. (All Bacteria)... 

Species Length of Amplicon (bp) Weak Signals May Also Occur for Enzyme Characteristic Restriction Fragments (bp)... 

There are also commercially available real-time PCR kits for celery detection, such as SureFood Allergen Celery from R-Biopharm. Generally, there is no information from PCR kit suppliers about the target for the assays, including the length of the amplicons. Information on the limit of detection in foods related to the use of a clearly specified reference material is also often missing. 

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