Antibacterial activity amphiphilic polymers with

Amphiphilic Polymers with Potent Antibacterial Activity, Chapter 11... 

Peptides exhibit the highest antimicrobial activities of amphiphilic polymers and also possess antibacterial, antiviral, antifungi and anticancer activities [30-32]. In view of the potential applications of peptides, we will now discuss the synthesis of some important antibacterial peptides. Gad-1 and Gad-2 are peptides with amino acid chains and are prepared using O-fluorenylmethyloxycarbonyl (Fmoc) chemistry. 

To measure the antibacterial activities of amphiphilic cationic polynorbornene derivatives, they developed a slightly different method based on optical density (OD). In this method, bacteria were grown in suspensions (Escherichia coli D31 and Bacillus subtilis ATCC 8037 ) of Miieller-Hinton broth (MHB) overnight at 37 °C, diluted with fresh MHB to an OD of 0.1 at 600 nm and further diluted by a factor of 10. This suspension was mixed with different concentrations of freshly prepared polymer solutions in rm-buffered saline (pH 6.5-7.0), by serial dilution in a 96-well plate, and incubated for 6 h at 37 °C. The OD 600 nm was measured for bacteria suspensions that were incubated in the presence of a polymer solution or fm-buffered saline only. After 6 h, the MIC for 90% inhibition of growth was determined. 

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