3-Aminophenyl phosphate hydrolysis

The hydrolysis of phosphates is discussed in reviews of nucleophile10 and secondary6 isotope effects. The kinetics and mechanism of the acid hydrolysis of 3-aminophenyl phosphate were studied in 0.1-0.6 m HC1 at 90 °C.55... 

The amplification of the amperometric response of redox species has been studied for p-aminophenol The sensitive detection of this compound is of particular interest since it is the basis for the measurement of enzymes such as alkaline phosphatase and (J-galactosidase, which are frequently used in enzyme immunoassays The electrode active species (p-aminophenol) is liberated in the enzyme catalysed hydrolysis of its aminophenylated substrate which itself exhibit an irreversible electrochemical behavior The utility of p-aminophenyl phosphate in enzyme immunoassays in combination with IDA electrodes has been demonstrated in [6] The unfavorable pH-optimum of alkaline phosphatase is the drawback of this system Because of its neutral pH-optimum p-galactosidase appeared to be more advantageous An illustration of the proposed electrochemical enzyme immunoassay is given in Fig 2... 

The rat LD qS are 13, 3.6 (oral) and 21, 6.8 (dermal) mg/kg. Parathion is resistant to aqueous hydrolysis, but is hydroly2ed by alkah to form the noninsecticidal diethjlphosphorothioic acid and -nitrophenol. The time required for 50% hydrolysis is 120 d ia a saturated aqueous solution, or 8 h ia a solution of lime water. At temperatures above 130°C, parathion slowly isomerizes to 0,%diethyl 0-(4-nitrophenyl) phosphorothioate [597-88-6] which is much less stable and less effective as an insecticide. Parathion is readily reduced, eg, by bacillus subtilis ia polluted water and ia the mammalian mmen to nontoxic 0,0-diethyl 0-(4-aminophenyl) phosphorothioate, and is oxidized with difficulty to the highly toxic paraoxon [511-45-5] diethyl 4-nitrophenyl phosphate d 1.268, soluble ia water to 2.4 mg/L), rat oral LD q 1.2 mg/kg. 

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