Aminoalcohols and Quaternary Ammonium Salts

Detection, The alkaloid reagents (Nos. 98, 147, 245, for example) have been those principally used for detecting aminoalcohols and quaternary ammonium salts. The dipicrylamine reagent (No. 90) is 

Desimio [5] has reported that biogenic amines can be separated on silica gel 6 layers, using the solvent mixture, commonly employed in PC, of n-butanol-acetic acid-water (66 -f 17 + 17). 6-Hydroxycatechol amines and metabolites can be separated also with the same solvent or with n-butanol-25% ammonium hydroxide-ethyl acetate (60 + 20 + 20) [4a]. 

Seileb and Wiechmann [35] separate mescaline and other phenyl-ethylamines on silica gel 6 layers with isopropanol-chloroform-concen-trated ammonium hydroxide (80 + 5 + 15). Through condensation with formaldehyde in the presence of concentrated ammonium hydroxide and subsequent heating with hydrochloric acid or with the Proch4zka reagent (No. 123), derivatives are obtained which show intense green fluorescence, adaptable to fluorometric quantitative determination or to further separation with ethyl acetate-methanol-formic acid (60 + 35 + 5 or 80 + 10 + 10) (limit of detection for mescaline = 0.01 (xg). 

A good separation of catechol amines and their metabolites is possible also on cellulose MN 300 layers, using various solvents [31] n-butanol, saturated with 3N hydrochloric acid, is suitable. The following hJ /-values are quoted for a 15 cm run  

Beckett and Choijlis [2], using cellulose, silica gel 6 and alumina G layers and the solvents n-butanol-acetic acid-water (40 + 10 + 50) or water-saturated n-butanol, separate histamine, noradrenaline, adrenaline, isoprenaline, ephedrine and jS-phenylethylamine. 

---