Sugars, amino, in polysaccharides

Rebers, P. A., Wessman, G. E., and Robyt, J. F. (1986). A thin-layer-chromatographic method for analysis of amino sugars in polysaccharide hydrolyzates. Carbohydr. Res. 153 132-135. 

Most of the polysaccharides of interest in this text are termed glycosaminoglycans, polymers that contain an amino sugar in the repeat unit. Glycosaminoglycans that are abundant in mammalian tissues include hyaluronan, chondroitin sulfate, dermatan sulfate, keratan sulfate, and heparin-heparan sulfate (see Table 2.4). Most of these glycosaminoglycans,... 

Modification of this procedure was applied to the analysis of neutral and amino sugars in mucins [440]. A mixture of 0.2% of ethylene glycol succinate, 0.2% of ethylene glycol adipate and 1.4% of silicone XE-60 was used as the stationary phase and the analysis was effected with temperature programming at l°C/min from 150 to 205°C. Similar results could be obtained, however, on a column packed with 3% of OV-225. Partially ethylated alditol acetates were used to determine the components of polysaccharides [441],... 

The importance of amino sugars in relation to soil polysaccharide is... 

The Jordi polyamine column is a polar column for simple sugar and polysaccharide applications. The amine groups are bonded to the DVB backbone and are stable in aqueous mobile phases. This material does not self-hydrolyze as do many silica-based amino packings (Fig. 13.14). 

FIGURE 1.9 (a) Amino acids build proteins by connecting the n-carboxyl C atom of one amino acid to the n-amino N atom of the next amino acid in line, (b) Polysaccharides are built by combining the C-1 of one sugar to the C-4 O of the next sugar in the polymer, (c) Nucleic acids are polymers of nucleotides linked by bonds between the 3 -OH of the ribose ring of one nucleotide to the 5 -P04 of its neighboring nucleotide. All three of these polymerization processes involve bond formations accompanied by the elimination of water (dehydration synthesis reactions). 

In tandem with his studies of polysaccharides, Stacey had a research group investigating the chemistry of the unusual sugars found as components of carbohydrate macromolecules. In this context, derivatives of simple sugars, di- and trisaccharides, uronic acids, deoxy sugars, amino sugars, sugar sulfates, and phosphates were studied. 

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