Alphastat regulation

Because this kind of role for histidyl residues is present in diverse types of binding sites, two major benefits of alphastat regulation are evident (Somero, 1986). One benefit is that enzymatic activity can be responsive to changes in pH . Shifts in pH during activity or in concert with transition between life stages are able to effectively titrate the activities of enzymes, to ensure that their function occurs in the appropriate direction and at an appropriate level. A second and closely related benefit is the preservation of reversibility in enzymatic reactions, as seen in the pH effects on Km values of LDH. 

What underlies the effect of pH on membrane fluidity and phase state The effect of an alphastat pH regimen on membrane phospholipids differs fundamentally from the influence that alphastat regulation has on protein... 

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