Allele-specific oligonucleotide

Saiki, R. K., Chang, C., Levenson, C. H., Warren, T. C., Boehm, C. D. etal.. Diagnosis of sickle cell anemia and (3-thalassemia with enzymatically amplified DNA and non radioactive allele-specific oligonucleotide probes. N. Engl. J. Med. 319, 537-541 (1988). 

Allele-specific oligonucleotide Specific alleles of a gene Genetic counseling and DNA fingerprinting... 

Figure 1-7-8. Comparison of Probes Single-Gene Versus Allele-Specific Oligonucleotide (ASO)...

For additional discussion of allele-specific oligonucleotide (ASO) probes and dot blots, see Section I, Chapter 7 Genetic Testing. 

Saiki, R., Bugawan, T.L., Horn, G.T., Mullis, K.B., Ehrlich, H.A. (1986) The analysis of enzymatically labeled / -globin DQa DNA with allele-specific oligonucleotide probes. Nature 324, 163-166. 

Techniques used to analyze DNA, RNA, and proteins. ASO = allele-specific oligonucleotides. ELISA = enzyme-linked immunosorbent assays. 

D. Dot blot using allele specific oligonucleotide probes... 

M.S. Ristaldi, M. Pirastu, C. Rosatelli, G. Monni, H. Erlich, R. Saiki, A. Cao, Prenatal Diagnosis of Beta-thalassaemia in Mediterranean Populations by Dot Blot Analysis with DNA Amplification and Allele Specific Oligonucleotide Probes , Prenat. Diagn, 9(9), 629-638 (1989). 

The basis of the allele-specific oligonucleotide (ASO) assay is that DNA duplexes which contain a mismatch are destabilized and have a lower melting temperature than correctly paired duplexes. To test for mutations using ASO, two probes, one containing the normal sequence and one containing the mutant sequence, are produced and hybridized to the patient s DNA. For each normal and mutant probe, conditions can be found where the probe will hybridize to only its perfectly matched duplex. If the patient sample contains only normal sequence, only the normal probe will hybridize. In a heterozygous sample, both the mutant and normal probes will hybridize, and in a homozygous mutant sample only the mutant probe will hybridize. 

Saiki RK, Bugawan TL, Horn GT, Mullis KB, Erlich HA. Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes. Nature 1986 324 163-166. 

DNA Isolation Techniques for Allele-Specific Oligonucleotide Assay or Gel Denaturing and Polymerase Chain Reaction... 

Fig. 2. Analysis of parental and hybrid maize rRNAs with allele-specific oligonucleotide probes. In the cross illustrated the maternal parent P-1 is B73 and the paternal parent P-2 is Mo 17. The samples were applied at 0.5 /ig/slot with six replicates of each. The parental and hybrid samples are identified. (A) the oligonucleotide probe used is the same one shown in Fig. 1A. In (B) the probe is the same as in Fig. IB.

Green ETC, Bain SC, Day PJ, Barnett AH, Charleson F, Jones AF, Walker MR. Detection of human apolipoprotein E3, E2, and E4 genotypes by an allele-specific oligonucleotide-primed polymerase chain reaction assay development and validation. Clin Chem 1991 37 1263-8. 

Weisgraber KH, Newhouse YM, Mahley RW. Apolipoprotein E genotyping using the polymerase chain reaction and allele-specific oligonucleotide probes. Biochem Biophys Res Commun 1988 157 1212-17. 

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