Alkane oxidation gene

In other bacteria, such as Acinetobacter, alkane oxidation genes are chromosomal (Singer Finnerty, 1984b). Moreover, some of the genes that participate in alkane oxidation probably comprise indispensable constituents of the bacterial cell. For example, in P. oleovorans, one of the gene products that catalyzes the initial oxidation of aliphatic substrates, the alkane hydroxylase, is an integral cytoplasmic membrane protein, and constitutes after induction 1.5-2% of the total cell protein (Nieboer et al. 1993). 

Fig. 14.4.1.1. Schematic diagram of a two-phase bioreactor system for continuous 1-octanol production. [After reference 7]. 1 -Octanol i s produced from n-octane in hexadecene by Pseudomonas oleovorans or recombinant strains containing the alkane oxidation genes.

Nieboer, M., Kingma, J. Witholt, B. (1993). The alkane oxidation system of Pseudomonas oleovorans induction of the alk genes in Escherichia colt W3110 (pGEc47) affects membrane biogenesis and results in overexpression of alkane hydroxylase in a distant cytoplasmic membrane subfraction. Molecular Microbiology, 8, 1039-51. 

Smits THM, Witholt B, van Beilen JB (2003) Functional characterization of genes involved in alkane oxidation by Pseudomonas aeruginosa. Anton Leeuw Int J G Mol Micro 84 193-200... 

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