Alkaline protease from Pseudomonas

The first structures of this kind were reported in 1993 pectate lyase G from Erwinia chrysanthemi (Yoder et al, 1993) and alkaline protease from Pseudomonas aeruginosa (Baumann et al, 1993). Based on consideration of these crystal structures, the term parallel //-helix was introduced for a fold containing three //-strands per coil, and parallel //-roll for a fold with two //-strands per coil (Baumann etal, 1993 Yoder andjurnak, 1995 Yoder et al., 1993). The epithet parallel was intended to emphasize the distinction between these folds and the previously observed helical structure of the antibiotic gramicidin which contains both l- and D-amino acids and... 

Louis, D., Kohlmann, M. and Wallach, J. (1997) Spectrophotometric assay for amidolytic activity of alkaline protease from Pseudomonas aeruginosa. Anal. Chim. Acta, 345, 219-225. 

Bhoopathy, N. R., Indhuja, D., Shrinivasan, K., Uthirappan, M., Gupta, R., Ramudu, K. N., Chellan, R. Statistical medium optimization of an alkaline protease from Pseudomonas aeruginosa MTCC 10501, its characterization and apphcation in leather processing. Indian J Expt Biol 2013, 51, 336-342. 

Similar to Figure 1, the palmitoyl (C-16) group has also been enzymatically grafted onto HEC. The reaction entails the incubation of HEC, vinyl palmitate, and a suitable enzyme in N,N-dimethylacetamide (DMAc) at 50°C. Several enzymes were attempted satisfactory results were obtained with Pseudomonas fluorescens lipase (Amano P-30), Pseudomonas capecia lipase (Lipase PS), or Alcalase immobilized alkaline protease from Novozymes A/S as a catalyst. Note that in this case a polar aprotic solvent (DMAc) is used, and one of the reactants, vinyl palmitate, has a fricile leaving group (vinyl alcohol which leaves as acetaldehyde). 

Kulkarni N, Gadre RV (1999) A novel alkaline, thermostable, protease-free lipase from Pseudomonas sp. Biotechnol Lett 21 897-899... 

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