Aldoses liquid chromatography

The melting temperatures of these derivatives are easily determined and are characteristic for each osazone. This information was used to help identify monosaccharides before the development of HPLC or gas-liquid chromatography. Listed below are the melting points (MPs) of some aldose-osazone derivatives ... 

There have been several reports of the separation of a- and / -pyranose forms during liquid chromatography of sugars.46-49 Initially, this was regarded as a nuisance, but systematic attempts were later made to improve this separation. At 0°, with 1 4 water-acetonitrile as the eluant, many aldoses were separated into two components.48 Galactose and al-trose showed small peaks probably representing the furanose forms ... 

High-Pressure Liquid Chromatography. - A new sensitive, selective amperometric detector has been developed, based on the oxidation in alkaline solutions of carbohydrates on a glassy carbon electrode onto which has been cast copper/copper oxide particles dispersed in a Nafion film. This system substantially lowers the overpotential for oxidation. Its use was demonstrated for the quantitation of various aldoses, sugar alcohols, and disaccharides in h.p.l.c. and flow injection analyses. ... 

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