Alanine myoglobins

In the case of His/CN systems, structurally pertinent to myoglobin cyanide, peroxidase cyanide, cytochrome c cyanide and the CN derivative of a cytochrome c mutant, where the axial ligand methionine is substituted with alanine (Ala80-cyt c-CN), the simple assignment of the four methyl protons, which is an almost trivial task nowadays, provides direct structural information on the axial ligands, which can be used for structural analysis in solution. The chemical shifts for each... 

Cytochrome c, lysozyme, myoglobin, ribonuclease A C18, etched and modified column diol open tubular column 30 mMphosphate, pH 2.14, 30 mM citric acid, pH 3-25 mM p-alanine, 30 mM acetic acid, pH 4.14 450 mm x 50 pm i.d. 250 mm packed length 103... 

Although CK and LD isozymes and myoglobin are sensitive biomarkers of skeletal muscle injury, they are also found in cardiac muscle and thus are not tissue specific. Cardiotoxicity must be excluded in order to use them effectively as biomarkers. Injured muscle releases other enzymes in significant amounts, such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and aldolase however, these also lack tissue specificity. 

Fig. 3. Elution profile of solution of lyophilized earthworm wash separated on a 1.6 x 42.5 cm column of AcA 44 using 0.15 M NaCl as eluent. Two ml fractions were collected. - - 17.6 mg EWV7 in 2 ml 0.15 M NaCl 0-0-0 21.3 mg EWW in 2 ml 0.15 M NaCl. DB = Blue Dextran ALB = bovine serum albumin OVA = ovalbumin MYO = myoglobin DNPA = DNP-alanine.

Hydrophobic side chains of phenylalanine, alanine, valine, leucine, isoleucine, and methionine are clustered in the interior of the molecule, where they are shielded from contact with water. Hydrophobic interactions are a major factor in directing the folding of the polypeptide chain of myoglobin into this compact, three-dimensional shape. 

ALAD = alanine dipeptide BPTI = bovine pancreatic trypsin inhibitor LJ = Lennard-Jones MbCO = carboxy-myoglobin NMA = A -methylacetamide vdW = van der Waals. 

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